Coding

Part:BBa_K5226039:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-10)


cysNC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 532
    Illegal SapI.rc site found at 600


Design Notes

none


Source

PCR amplification.Sequence source is from Halomonas sp. TD01 strain, found through NCBI(https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=2677331&to=2679235)

References